Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.

AUTOR(ES)

Russel, M

RESUMO

To determine whether the fip gene of Escherichia coli, which is required for filamentous phage assembly, is required for cell viability, we replaced the chromosomal copy of the gene with an inactive copy introduced on a plasmid. We found that the fip gene is dispensable. The method we devised, which should be generally useful, was also tested with an inactivated rho gene. As expected, the rho gene is essential.

Documentos Relacionados

• Liposome-mediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid.
• Influence on motility of Escherichia coli and Salmonella typhimurium by a naturally occurring conjugative plasmid.
• Modification of DNA by glucose 6-phosphate induces DNA rearrangements in an Escherichia coli plasmid.
• mprA, an Escherichia coli gene that reduces growth-phase-dependent synthesis of microcins B17 and C7 and blocks osmoinduction of proU when cloned on a high-copy-number plasmid.
• Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.