Replication dependent and cell specific activation of the polyomavirus early promoter.

AUTOR(ES)

Yoshimoto, K K

RESUMO

The relationship of viral DNA replication to the activation of viral gene expression is usually considered with respect to late genes. In this report we examine the replication activation of the polyomavirus early promoter. Using origin active and inactive mutants to drive luciferase gene expression from the polyomavirus early promoter, we show that the early promoter is also subjected to a replication dependent activation. The degree of activation can be up to a hundred fold greater than that seen without replication and is about 13 fold on a per template basis. This replication based activation is, however, cell type dependent and was seen in FOP cells but not in 3T6 cells. Analysis of the requirements of cis acting DNA show that these enhancer elements affect early transcription predominantly through the activation of replication, although some replication independent stimulation can also be seen. The implications of this result for the regulation of polyomavirus early gene regulation are considered.

Documentos Relacionados

• Replication-dependent transactivation of the polyomavirus late promoter.
• Activation domains of transcription factors mediate replication dependent transcription from a minimal HIV-1 promoter.
• Specific cellular proteins bind to critical promoter sequences of the adenovirus early EIIa promoter.
• A cell type specific factor recognizes the rat thyroglobulin promoter.
• Cell-specific regulation of oncogene-responsive sequences of the c-fos promoter.