Replication fork collapse at replication terminator sequences

AUTOR(ES)

Bidnenko, Vladimir

FONTE

Oxford University Press

RESUMO

Replication fork arrest is a source of genome re arrangements, and the recombinogenic properties of blocked forks are likely to depend on the cause of blockage. Here we study the fate of replication forks blocked at natural replication arrest sites. For this purpose, Escherichia coli replication terminator sequences Ter were placed at ectopic positions on the bacterial chromosome. The resulting strain requires recombinational repair for viability, but replication forks blocked at Ter are not broken. Linear DNA molecules are formed upon arrival of a second round of replication forks that copy the DNA strands of the first blocked forks to the end. A model that accounts for the requirement for homologous recombination for viability in spite of the lack of chromosome breakage is proposed. This work shows that natural and accidental replication arrests sites are processed differently.

Documentos Relacionados

• Reorganization of terminator DNA upon binding replication terminator protein: implications for the functional replication fork arrest complex.
• Replication Terminator Protein-Based Replication Fork-Arrest Systems in Various Bacillus Species
• Swi1 Prevents Replication Fork Collapse and Controls Checkpoint Kinase Cds1
• Flanking sequences affect replication arrest at the Escherichia coli terminator TerB in vivo.
• Nucleosome positioning at the replication fork