Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.
AUTOR(ES)
Murti, K G
RESUMO
Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed: (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that: (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=370009Documentos Relacionados
- Macronuclear gene-sized molecules of hypotrichs.
- Gene-sized DNA molecules of the Oxytricha macronucleus have the same terminal sequence.
- In vitro aggregation of the gene-sized DNA molecules of the ciliate Stylonychia mytilus.
- Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.
- Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.