Replication of the Escherichia coli Chromosome Following Thymine Starvation
AUTOR(ES)
Liebart, J. C.
RESUMO
The replication of the chromosome of E. coli 15T- (thymine-requiring) has been followed for approximately four generations after a premature initiation of DNA synthesis by thymine starvation. Various labelings of the DNA with 14C, 3H, and density media allowed discrimination of the strands preexistent to thymine starvation from those which were made during the subsequent generations. Results were: (1) There is very little, if any, DNA degradation following 45 min of thymine starvation. (2) The growing points which existed prior to thymine starvation proceeded to the end of the chromosomes after resumption of DNA synthesis and then disappeared. (3) The premature DNA synthesis initiated by thymine starvation involved, symmetrically, all free origins; the entire chromosomal population was subjected to this process. (4) All the DNA which was synthesized during the progression of the premature growing points was normally replicated, i.e., with no delay or loss, during the next three generations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388474Documentos Relacionados
- Loss of Deoxyribonucleic Acid-Thymine During Thymine Starvation of Escherichia coli
- Technique for starvation of Escherichia coli of thymine.
- Influence of Thymine Starvation on the Integrity of Deoxyribonucleic Acid in Escherichia coli
- Inititation and termination of chromosome replication in Escherichia coli subjected to amino acid starvation.
- Control of Cell Division in Escherichia coli: Experiments with Thymine Starvation
