Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.

AUTOR(ES)

Singh, K K

RESUMO

Saccharomyces cerevisiae responds to DNA damage by arresting cell cycle progression (thereby preventing the replication and segregation of damaged chromosomes) and by inducing the expression of numerous genes, some of which are involved in DNA repair, DNA replication, and DNA metabolism. Induction of the S. cerevisiae 3-methyladenine DNA glycosylase repair gene (MAG) by DNA-damaging agents requires one upstream activating sequence (UAS) and two upstream repressing sequences (URS1 and URS2) in the MAG promoter. Sequences similar to the MAG URS elements are present in at least 11 other S. cerevisiae DNA repair and metabolism genes. Replication protein A (Rpa) is known as a single-stranded-DNA-binding protein that is involved in the initiation and elongation steps of DNA replication, nucleotide excision repair, and homologous recombination. We now show that the MAG URS1 and URS2 elements form similar double-stranded, sequence-specific, DNA-protein complexes and that both complexes contain Rpa. Moreover, Rpa appears to bind the MAG URS1-like elements found upstream of 11 other DNA repair and DNA metabolism genes. These results lead us to hypothesize that Rpa may be involved in the regulation of a number of DNA repair and DNA metabolism genes.

Documentos Relacionados

• Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes.
• Cotyledon nuclear proteins bind to DNA fragments harboring regulatory elements of phytohemagglutinin genes.
• The purification of an erythroid protein which binds to enhancer and promoter elements of haemoglobin genes.
• The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes.
• Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer.