Requirement for a fluid host cell membrane in injection of coliphage T5 DNA.
AUTOR(ES)
Labedan, B
RESUMO
Injection of T5 first-step-transfer DNA was prevented at 29 degrees C, after adsorption to an unsaturated fatty acid mutant grown on elaidate (phase transition at 35 degrees C). Local anesthetics, which increase membrane fluidity, did not inhibit injection above transition temperature and could even reverse the inhibition observed at 29 degrees C on elaidate cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255453Documentos Relacionados
- Host cell metabolic energy is not required for injection of bacteriophage T5 DNA.
- Requirement for membrane potential in injection of phage T4 DNA.
- Distribution of "minor" nicks in bacteriophage T5 DNA.
- New physical map of bacteriophage T5 DNA.
- Role of the T5 gene D15 nuclease in the generation of nicked bacteriophage T5 DNA.
