Requirement of Varicella-Zoster Virus Immediate-Early 4 Protein for Viral Replication
AUTOR(ES)
Sato, Bunji
FONTE
American Society for Microbiology
RESUMO
Varicella-zoster virus (VZV) is an alphaherpesvirus that causes two diseases, chickenpox and zoster. VZV open reading frame 4 (ORF4) encodes the immediate-early 4 (IE4) protein, which is conserved among alphaherpesvirus and has transactivation activity in transient transfections. To determine whether the ORF4 gene product is essential for viral replication, we used VZV cosmids to remove ORF4 from the VZV genome. Deleting ORF4 was incompatible with recovery of infectious virus, whereas transfections done by using repaired cosmids with ORF4 inserted at a nonnative site yielded virus. To analyze the functional domain of IE4, we introduced a mutation altering the C-terminal amino acids, KYFKC (K443S), which was designed to disrupt the dimerization of IE4 protein. Transfections with these mutant cosmids yielded no virus, indicating that this KYFKC motif was essential for IE4 function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254250Documentos Relacionados
- Mutational analysis of varicella-zoster virus major immediate-early protein IE62.
- Varicella-zoster virus open reading frame 4 encodes an immediate-early protein with posttranscriptional regulatory properties.
- The varicella-zoster virus immediate-early protein IE62 is a major component of virus particles.
- Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency.
- Variability of Immediate-Early Gene 62 in German Varicella-Zoster Virus Wild-Type Strains ▿
