Response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization.

AUTOR(ES)

Offit, P A

RESUMO

Sera from (i) gnotobiotic BALB/c, CD-1, and CFW mice and (ii) conventional BALB/c mice were evaluated by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization, using the Wa, SA-11, and WC-3 (bovine) strains of rotavirus as the detecting antigens. The gnotobiotic mice had no antirotavirus antibody detectable by radioimmunoprecipitation and no neutralizing antibody at a dilution of 1:50 by plaque reduction neutralization. All sera from the conventional mice had rotavirus-specific antibodies detected by radioimmunoassay and by radioimmunoprecipitation at serum dilutions of 1:50 and 1:10,000, respectively. The antibodies were directed against viral proteins p116, p94, p88, and p84 of all three viruses, but had no neutralizing activity against heterologous rotaviruses at a dilution of 1:50. Conventional seropositive mice were parenterally immunized with the Wa, SA-11, or WC-3 strain of rotavirus. An approximate 100-fold increase in rotavirus-specific antibodies was detected by radioimmunoassay, and greater than 20-fold selective neutralization of the immunizing strain of virus was observed. Sera from the mice immunized with Wa virus had antibodies directed against inner and outer capsid proteins of all three rotaviruses. The mouse can be a useful model for studying the immune response to heterologous rotavirus infection; preexisting antibodies presumably directed towards murine rotavirus do not prevent the development of a type-specific immune response to a nonmurine rotavirus.

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