Retroviral expression of the human IL-2 gene in a murine T cell line results in cell growth autonomy and tumorigenicity.
AUTOR(ES)
Yamada, G
RESUMO
In mature T lymphocytes (T cells) the regulated expression of the genes for interleukin-2 (IL-2) and its receptor (IL-2R) constitutes an essential part in controlling the cell growth. Evidence has been provided which suggests the involvement of an aberrant function of the IL-2 system in developing T cell neoplasms, particularly the adult T cell leukemia/lymphoma (ATL). As an approach to examine the extent of the IL-2 system contribution to T cell neoplasms, we created the experimental conditions wherein both IL-2 and IL-2R are expressed constitutively in a murine T cell line. We made use of a retroviral vector to infect an IL-2-dependent CTLL-2 line and lead to the expression of human IL-2. Here, we show that the virus-infected cells not only proliferate in vitro in the absence of exogenously supplied IL-2 under certain conditions, but also develop tumors (lymphomas) in nude and syngeneic mice.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553693Documentos Relacionados
- Expression of murine interleukin 7 in a murine glioma cell line results in reduced tumorigenicity in vivo.
- Transfection of the int-1 mammary oncogene in cuboidal RAC mammary cell line results in morphological transformation and tumorigenicity.
- Involvement of integrin alpha V gene expression in human melanoma tumorigenicity.
- p21ras mediates control of IL-2 gene promoter function in T cell activation.
- Identification of multiple cis-elements and trans-acting factors involved in the induced expression of human IL-2 gene.