Reverse Gyrase Is Not a Prerequisite for Hyperthermophilic Life
AUTOR(ES)
Atomi, Haruyuki
FONTE
American Society for Microbiology
RESUMO
We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90°C. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=438624Documentos Relacionados
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