Riboregulation in Escherichia coli: DsrA RNA acts by RNA:RNA interactions at multiple loci
AUTOR(ES)
Lease, Richard A.
FONTE
The National Academy of Sciences
RESUMO
DsrA is an 87-nt untranslated RNA that regulates both the global transcriptional silencer and nucleoid protein H-NS and the stationary phase and stress response sigma factor RpoS (σs). We demonstrate that DsrA acts via specific RNA:RNA base pairing interactions at the hns locus to antagonize H-NS translation. We also give evidence that supports a role for RNA:RNA interactions at the rpoS locus to enhance RpoS translation. Negative regulation of hns by DsrA is achieved by the RNA:RNA interaction blocking translation of hns RNA. In contrast, results suggest that positive regulation of rpoS by DsrA occurs by formation of an RNA structure that activates a cis-acting translational operator. Sequences within DsrA complementary to three additional genes, argR, ilvIH, and rbsD, suggest that DsrA is a riboregulator of gene expression that acts coordinately via RNA:RNA interactions at multiple loci.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=22852Documentos Relacionados
- A trans-acting RNA as a control switch in Escherichia coli: DsrA modulates function by forming alternative structures
- The Small Noncoding DsrA RNA Is an Acid Resistance Regulator in Escherichia coli†
- Hfq Is Necessary for Regulation by the Untranslated RNA DsrA
- Temperature Sensing by the dsrA Promoter
- The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli.