RNA aggregation during sulfhydryl-agarose chromatography of mercurated RNA.

AUTOR(ES)

Konkel, D A

RESUMO

Isolation of newly synthesized mercurated RNA transcripts by chromatography on sulfhydryl-agarose has recently been used to reduce contamination by endogenous RNA derived from the chromatin template. We show that substantial RNA aggregation occurs during standard isolation procedures, causing significant retention of endogenous (unmercurated) RNA on sulfhydryl-agarose. We describe methods to reduce substantially this problem and discuss the implications of our findings for interpretation of previous hybridization and transcription experiments.

Documentos Relacionados

• Mapping of adenovirus late promoters with nascent mercurated RNA.
• Mercurated Dextran Column Chromatography for Fractionating Mononucleotides
• Phosphoryl migration during the chemical synthesis of RNA.
• A non-radioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands.
• Purification of the Rous sarcoma virus src kinase by casein-agarose and tyrosine-agarose affinity chromatography.