RNA nicking activity associated with DNA ligase of T4 infected E. coli: properties and influence on in vitro reactions of ligase.
AUTOR(ES)
Sano, H
RESUMO
Highly purified DNA ligase from T4 infected E. coli displays an RNA nicking activity which cleaves endonucleolytically the RNA of ribo-desoxy-and ribo-ribo type doublestranded structures to oligonucleotides with 5'phosphoryl-and 3'hydroxy termini. In the presence of ATP the generated nicks are repaired by the ligase except at the ends of the doublestranded regions where some short oligonucleotides are released before ligation can occur. As judged from its behaviour during the various purification steps and from some of its properties, the nicking activity seems to be different from known nicking enzymes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343527Documentos Relacionados
- Reactions at the termini of tRNA with T4 RNA ligase.
- In vitro selection of optimal DNA substrates for T4 RNA ligase.
- Equimolar addition of oligoribonucleotides with T4 RNA ligase.
- A novel DNA joining activity catalyzed by T4 DNA ligase.
- Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.
