RNA sequences complementary to citrus exocortis viroid in nucleic acid preparations from infected Gynura aurantiaca
AUTOR(ES)
Grill, L. K.
RESUMO
Molecular hybridization with 125I-labeled citrus exocortis viroid RNA has been used to survey nucleic acid preparations from Gynura aurantiaca for viroid complementary molecules. A differential hybridization effect was detected between nucleic acid extracts from healthy and infected tissue in which significant RNase-resistant 125I-labeled citrus exocortis viroid resulted in hybridization studies with the infected tissue extracts. Subsequent characterization indicated that RNA from infected tissue was involved in the formation of a duplex molecule with citrus exocortis viroid RNA and had properties of an RNA·RNA hybrid. Subcellular fractionation of infected tissue indicates that the complementary RNA is present in nuclear and soluble RNA fractions. This RNA may represent an intermediate molecule in the replication of the viroid or a pathogenic expression and may have a regulatory role in the host cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411364Documentos Relacionados
- The sequence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid.
- Videiras duplamente infectadas pelo Citrus exocortis viroid e Hop stunt viroid no Brasil
- Correlation between Ornithine Decarboxylase and Putrescine in Tomato Plants Infected by Citrus Exocortis Viroid or Treated with Ethephon.
- Properties of a cell-free system for synthesis of citrus exocortis viroid
- Nuclear DNA from uninfected or potato spindle tuber viroid-infected tomato plants contains no detectable sequences complementary to cloned double-stranded viroid cDNA.