Role for a Phage Promoter in Shiga Toxin 2 Expression from a Pathogenic Escherichia coli Strain
AUTOR(ES)
Wagner, Patrick L.
FONTE
American Society for Microbiology
RESUMO
Shiga toxins (Stxs), encoded by the stxA and stxB genes, are important contributors to the virulence of Escherichia coli O157:H7 and other Stx-producing E. coli (STEC) strains. The stxA and stxB genes in STEC strains are located on the genomes of resident prophages of the λ family immediately downstream of the phage late promoters (pR′). The phage-encoded Q proteins modify RNA polymerase initiating transcription at the cognate pR′ promoter which creates transcription complexes that transcend a transcription terminator immediately downstream of pR′ as well as terminator kilobases distal to pR′. To test if this Q-directed processive transcription plays a role in stx2AB expression, we constructed a mutant prophage in an O157:H7 clinical isolate from which pR′ and part of Q were deleted but which has an intact pStx, the previously described stx2AB-associated promoter. We report that production of significant levels of Stx2 in this O157:H7 isolate depends on the pR′ promoter. Since transcription initiating at pR′ ultimately requires activation of the phage lytic cascade, expression of stx2AB in STEC depends primarily on prophage induction. By showing this central role for the prophage in stx2AB expression, our findings contradict the prevailing assumption that phages serve merely as agents for virulence gene transfer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=95105Documentos Relacionados
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