Role of the Polyadenylate Segment in the Translation of Globin Messenger RNA in Xenopus Oocytes
AUTOR(ES)
Huez, G.
RESUMO
The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell-free extract of Krebs II ascites tumor. It is concluded that the presence of the 3′ poly(A)-rich sequence in mRNA is required to ensure high functional stability.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388638Documentos Relacionados
- Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
- Translation and stability of human globin mRNA in Xenopus oocytes.
- A Particle Associated with the Polyadenylate Segment in Mammalian Messenger RNA
- Elongation of the Polyadenylate Segment of Messenger RNA in the Cytoplasm of Mammalian Cells
- Phosphorylation of a 60 kDa polypeptide from Xenopus oocytes blocks messenger RNA translation.