Rous sarcoma virus direct repeat cis elements exert effects at several points in the virus life cycle.
AUTOR(ES)
Simpson, S B
RESUMO
Two approximately 135-nucleotide (nt) direct repeats flank the Rous sarcoma virus (RSV) oncogene src and are composed of two smaller repeats, dr1 (approximately 100 nt) and dr2 (approximately 36 nt). These sequences have been reported to contain cis-acting signals necessary for RNA packaging and elements that allow cytoplasmic accumulation of unspliced RNA (cytoplasmic transport elements). In this report, we show that avian fibroblasts infected with the Prague A strain of RSV with precise deletions of both dr1 elements express src and are transformed by this mutant virus but production of virus particles is very low and virus spread throughout the culture requires several weeks. We show that the replication defect is due to complex effects on viral RNA transport, viral RNA half-life, and virus particle assembly. The dr1 elements may contain binding sites for a permissive cell-specific factor(s) that facilitates efficient nuclear-cytoplasmic transport, RNA stability, and cytoplasmic utilization of unspliced viral RNA. The implications of these results for understanding the defects of nonpermissive virus infections in mammalian cells are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=230216Documentos Relacionados
- The 5′ and 3′ TAR Elements of Human Immunodeficiency Virus Exert Effects at Several Points in the Virus Life Cycle
- cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus.
- Selective Inhibition of Splicing at the Avian Sarcoma Virus src 3′ Splice Site by Direct-Repeat Posttranscriptional cis Elements
- A novel 165-base-pair terminal repeat sequence is the sole cis requirement for the adeno-associated virus life cycle.
- Identification of transcriptional elements within the long terminal repeat of Rous sarcoma virus.