rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift.
AUTOR(ES)
Larsen, B
RESUMO
Base pairing between the 3' end of 16S rRNA and mRNA is shown to be important for the programmed -1 frameshifting utilized in decoding the Escherichia coli dnaX gene. This pairing is the same as the Shine-Dalgarno pairing used by prokaryotic ribosomes in selection of translation initiators, but for frameshifting the interaction occurs within elongating ribosomes. For dnaX -1 frameshifting, the 3' base of the Shine-Dalgarno sequence is 10 nucleotides 5' of the shift site. Previously, Shine-Dalgarno rRNA-mRNA pairing was shown to stimulate the +1 frameshifting necessary for decoding the release factor 2 gene. However, in the release factor 2 gene, the Shine-Dalgarno sequence is located 3 nucleotides 5' of the shift site. When the Shine-Dalgarno sequence is moved to the same position relative to the dnaX shift site, it is inhibitory rather than stimulatory. Shine-Dalgarno interactions by elongating ribosomes are likely to be used in stimulating -1 frameshifting in the decoding of a variety of genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=197052Documentos Relacionados
- A programmed –1 ribosomal frameshift signal can function as a cis-acting mRNA destabilizing element
- Characterization of the frameshift stimulatory signal controlling a programmed –1 ribosomal frameshift in the human immunodeficiency virus type 1
- A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein.
- Partial rescue of human carbonic anhydrase II frameshift mutation by ribosomal frameshift.
- Adenine-guanine base pairing ribosomal RNA.
