Schedule-dependent cytotoxicity of methotrexate and 5-fluorouracil in human colon and breast tumor cell lines.
AUTOR(ES)
Benz, C
RESUMO
The effect of sequential methotrexate and 5-fluorouracil on the clonal growth of the human colon adenocarcinoma cell, HCT-8, and the hormone-dependent human breast carcinoma cell, 47-DN, was examined. In both cell lines, when 5-fluorouracil was given during the last 6 h of a 24 h methotrexate exposure period, there was marked synergistic inhibition of clonal growth. Shorter intervals or the reverse sequence of drugs were either additive or antagonistic. These results indicate the importance of the drug sequence and time interval between drug administration for optimal cytotoxicity in these human cell lines. This information suggests that the administration of methotrexate 18 h before 5-fluorouracil may have potential application in the design of clinical trials for these malignancies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=371555Documentos Relacionados
- DNA mismatch repair (MMR)-dependent 5-fluorouracil cytotoxicity and the potential for new therapeutic targets
- Eudragit-coated pectin microspheres of 5-fluorouracil for colon targeting
- Analysis of gene amplification in human tumor cell lines.
- Tumor necrosis factor expression in human epithelial tumor cell lines.
- Evaluation of the hyperthermotherapy associated with paclitaxel, 5-fluorouracil and 5-fluorouracil plus folinic acid on the Walker 256 tumor implanted in rat stomachs
