Screening for Barley Mutants With Altered Hormone Sensitivity in Their Aleurone Layers 1

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A method, based on the diffusion assay of α-amylase on agar plates, was developed to screen for barley (Himalaya) mutants with altered sensitivity to gibberellic acid (GA3) or abscisic acid (ABA) in their aleurone layers. The seeds produced by sodium azide-mutagenized barley were screened for their ability to synthesize and secrete α-amylase when treated with different combinations of hormones. Various GA3-insensitive or supersensitive, ABA-insensitive, temperature-dependent GA3-insensitive, and constitutive mutants have been identified. Several stable mutants with altered GA3 sensitivity were recovered. Two of the homozygous GA3-insensitive mutants have been preliminarily characterized. The GA3-enhanced production of α-amylase and release of phosphatase are hampered in these mutants. However, they have normal stem height, and the uptake of GA3 by their aleurone layers appears to be the same as that of wild-type barley. They are most likely regulatory mutants affecting both α-amylase synthesis and phosphatase release.

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