Secondary structure determination of the conserved 98-base sequence at the 3' terminus of hepatitis C virus genome RNA.
AUTOR(ES)
Blight, K J
RESUMO
The RNA genome of hepatitis C virus (HCV) terminates with a highly conserved 98-base sequence. Enzymatic and chemical approaches were used to define the secondary structure of this 3'-terminal element in RNA transcribed in vitro from cloned cDNA. Both approaches yielded data consistent with a stable stem-loop structure within the 3'-terminal 46 bases. In contrast, the 5' 52 nucleotides of this 98-base element appear to be less ordered and may exist in multiple conformations. Under the experimental conditions tested, interaction between the 3' 98 bases and upstream HCV sequences was not detected. These data provide valuable information for future experiments aimed at identifying host and/or viral proteins which interact with this highly conserved RNA element.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=192079Documentos Relacionados
- Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA.
- Secondary Structure of the 3′ Terminus of Hepatitis C Virus Minus-Strand RNA
- Structural characterization of the highly conserved 98-base sequence at the 3′ end of HCV RNA genome and the complementary sequence located at the 5′ end of the replicative viral strand
- Structure of the 3' terminus of the hepatitis C virus genome.
- RNA-protein interactions at the 3' end of the hepatitis A virus RNA.
