Selection of Escherichia coli K-12 chromosomal mutants that prevent expression of F-plasmid functions.

AUTOR(ES)

Silverman, P

RESUMO

Chromosomal mutants of Escherichia coli deficient in the expression of F-plasmid functions were selected by mutagenizing F- cells, introducing an F' plasmid into the mutagenized cells by conjugation, and identifying transconjugants resistant to the donor-specific bacteriophage Q beta by a simple spray test. All but 1 of 25 mutants were defective in an extracellular stage of Q beta infection, suggesting that they fail to elaborate F-pili. At least six of these were also deficient as deoxyribonucleic acid donors. More than half of the mutants appear to be altered in peviously undetected chromosomal genes required for the expression of F-related cellular functions.

Documentos Relacionados

• Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB.
• Nonrandom F-plasmid replication in Escherichia coli K-12.
• Nucleotide sequence of traQ and adjacent loci in the Escherichia coli K-12 F-plasmid transfer operon.
• Chromosomal mutations of Escherichia coli that alter expression of conjugative plasmid functions.
• Expression of R-plasmid functions during anaerobic growth of an Escherichia coli K-12 host.