Selection of primary cell cultures with cre recombinase induced somatic mutations from transgenic mice.

AUTOR(ES)

Zeh, K

RESUMO

Deletion of genes in defined cell types has been achieved using a combination of gene targeting techniques and the Cre- lox P recombination system. Here we present a method to selectively isolate genetically altered primary cell cultures based on the permanent activation of a drug-resistance gene by the Cre recombinase. Transgenic mice were generated harboring a dormant form of the hygromycin resistance gene. This mouse line was crossed with mice carrying a constitutive Cre gene and an endogenous floxed allele. Primary fibroblasts established from triple transgenic embryos displayed not only hygromycin resistance but also recombination of the endogenous floxed allele. These results prove the potential of this approach.

Documentos Relacionados

• Cre-mediated somatic site-specific recombination in mice.
• Temporal control of the Cre recombinase in transgenic mice by a tetracycline responsive promoter.
• p53 mutations are not selected for in simian virus 40 T-antigen-induced tumors from transgenic mice.
• Spectra of spontaneous and mutagen-induced mutations in the lacI gene in transgenic mice.
• Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus