SELECTIVE MEDIUM FOR CARBOHYDRATE-UTILIZING TRANSDUCTANTS OF STAPHYLOCOCCUS AUREUS

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Murphey, W. H. (The University of Texas, Dallas), and E. D. Rosenblum. Selective medium for carbohydrate-utilizing transductants of Staphylococcus aureus. J. Bacteriol. 87:1198–1201. 1964.—The composition and properties of modified eosin-methylene blue agar are described. It is an efficient, selective medium for isolation of carbohydrate-utilizing staphylococci from large, nonutilizing populations. The medium has been used to measure the rate of transduction of mannitol fermentation and to demonstrate genetic recombination in mannitol-negative mutants of Staphylococcus aureus.

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