Selective translation initiation on bicistronic simian virus 40 late mRNA.
AUTOR(ES)
Grass, D S
RESUMO
We described previously a simian virus 40 (SV40) mutant, pSVAdL, that was defective in synthesis of the late viral protein VP1. This mutant, which contains a 100-base-pair fragment of adenovirus DNA encompassing the major late promoter inserted in the SV40 late promoter region (SV40 nucleotide 294), efficiently synthesizes agnoprotein, a protein encoded by the leader region of the same mRNA that encodes VP1. When the agnoprotein AUG initiation codon in pSVAdL was mutated to UUG, agnoprotein synthesis was abolished, and VP1 synthesis was elevated to wild-type levels. Because levels of late mRNA synthesis were not affected by this mutation, these results support a scanning model of translation initiation and suggest that internal translational reinitiation does not occur efficiently in this situation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283703Documentos Relacionados
- Deletion mutants of simian virus 40 defective in biosynthesis of late viral mRNA.
- Translational control of synthesis of simian virus 40 late proteins from polycistronic 19S late mRNA.
- Characterization of the 5'-terminal capped structures of late simian virus 40-specific mRNA.
- In vitro splicing of simian virus 40 early pre mRNA.
- Secondary structure model for the complete simian virus 50 late precursor mRNA.
