Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster.

AUTOR(ES)

Kay, M A

RESUMO

We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribosomal protein. Furthermore, our results indicate that most ribosomal protein mRNAs are regulated similarly to rpA1 mRNA. This conclusion is based on cell-free translation of mRNAs derived from polysomes and postpolysomal supernatants as well as in vivo labeling experiments. Thus, the translation of many ribosomal protein mRNAs appears to be temporally related to the synthesis of rRNA during D. melanogaster development. The relationship between rRNA transcription and ribosomal protein mRNA translation was further investigated by genetically reducing rRNA synthesis with the use of bobbed mutants. Unexpectedly, neither ribosomal protein mRNA abundance nor translation was altered in these mutants.

Documentos Relacionados

• Expression of the disconnected gene during development of Drosophila melanogaster.
• Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver.
• Antisense ribosomal protein gene expression specifically disrupts oogenesis in Drosophila melanogaster.
• Expression of ras cellular oncogenes during development of Drosophila melanogaster.
• Dosage regulation of ribosomal DNA in Drosophila melanogaster.