Separation of Herpes Simplex Virus-Induced Antigens by Concanavalin A Affinity Chromatography
AUTOR(ES)
De Leon, Manuel Ponce
RESUMO
Biologically active herpes simplex virus (HSV)-induced antigens were selectively removed from extracts of infected BHK cells by affinity chromatography by utilizing an insoluble form of concanavalin A (Con A). Soluble extracts of 3H-glucosamine-labeled, HSV-infected cells were absorbed to a Con A column. Bound material was eluted with α-methyl-d-mannoside (αMM) and NaCl. The specific activity of the eluted glycoproteins increased by 10-fold. Two broad groups of viral-induced antigens were isolated from Con A. Group I includes two antigens which bind to Con A by a specific mechanism because the antigens are dissociated by αMM. Group II contains three antigens which bind to Con A but apparently by a nonspecific or electrolytic mechanism. One antigen in group I was identified as the glycoprotein antigen, CP-1, described previously.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356695Documentos Relacionados
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