Separation of the transcriptional coactivator and antirepression functions of transcription factor IIA.
AUTOR(ES)
MA, D
RESUMO
Human transcription factor IIA (TFIIA) is composed of three subunits (alpha, beta, and gamma). TFIIA interacts with the TATA-box binding protein and can overcome repression of transcription. TFIIA was found to be necessary for VP16-mediated transcriptional activation through a coactivator function. We have separated the coactivator and antirepression activities of TFIIA. A TFIIA lacking the alpha subunit was isolated from HeLa cells. This "mini-TFIIA" interacts with the TATA-box binding protein and can overcome repression of transcription, but it is defective in transcriptional coactivator function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=39068Documentos Relacionados
- A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA.
- Interaction of the human T-cell lymphotropic virus type 1 tax transactivator with transcription factor IIA.
- The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription
- Molecular analysis of the 3,6-dideoxyhexose pathway genes of Yersinia pseudotuberculosis serogroup IIA.
- SSRP1 functions as a co-activator of the transcriptional activator p63