Sequence and temporal appearance of the early transcribed baculovirus gene HE65.

AUTOR(ES)

Becker, D

RESUMO

We have identified the early transcribed HE65 gene by screening a cDNA library from polyadenylated RNA which was isolated at 1 h after infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus (AcNPV). Nucleotide sequencing analysis of the HE65-specific cDNA clone reveals one open reading frame of 1,662 nucleotides from which a protein of 65 kDa in size can be predicted. The HE65 gene is located downstream of the late transcribed p80 gene and upstream of the homologous region hr4left, which overlaps the 5' sequences of the HE65 gene. An HE65-specific transcript of about 1,800 nucleotides is detectable 2 h postinfection and remains stable during the late phases of infection. RNase protection and primer extension analyses demonstrate that transcripts from the early start site of HE65 continue to accumulate from 2 to 48 h postinfection, even in the presence of aphidicolin. Furthermore, transcriptional analysis of the HE65 gene indicates a lower intensity of early transcription in comparison with the very early transcribed genes IEN, PE38, and ME53.

Documentos Relacionados

• Identification of the very early transcribed baculovirus gene PE-38.
• Nucleotide Sequence and Temporal Expression of a Baculovirus Regulatory Gene
• Temporal regulation of baculovirus RNA: overlapping early and late transcripts.
• Baculovirus gene ME53, which contains a putative zinc finger motif, is one of the major early-transcribed genes.
• Sequence analysis of the transcribed and 5' non-transcribed regions of the ribosomal RNA gene in Dictyostelium discoideum.