Sequence and transcriptional regulation of com101A, a locus required for genetic transformation in Haemophilus influenzae.
AUTOR(ES)
Larson, T G
RESUMO
A 2.8-kb EcoRI-BglII fragment cloned from the wild-type Haemophilus influenzae Rd chromosome is shown to increase the transformability of the Com-101 mutant through trans complementation. Deletion and sequence analyses indicate that the active region of the clone carries a 687-bp open reading frame. A 0.3-kb insertion in the corresponding EcoRI-BglII fragment of the Com-101 chromosome is shown to be a partial (331-bp) duplication of this open reading frame. The wild-type sequence produces a peptide of a size that is consistent with the sequence data when this sequence is expressed in Escherichia coli with a T7 promoter-based transcription vector. RNA hybridization analysis using a DNA probe derived from the open reading frame suggests that the sequence is transiently expressed during competence development. On the basis of these observations, it is proposed that the open reading frame corresponds to the com101A gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=208145Documentos Relacionados
- Identification of a DNA transformation gene required for com101A+ expression and supertransformer phenotype in Haemophilus influenzae.
- Mechanism of additive genetic transformation in Haemophilus influenzae.
- Donor DNA processing is blocked by a mutation in the com101A locus of Haemophilus influenzae.
- Plasmid transformation in Haemophilus influenzae.
- Role of the electrochemical proton gradient in genetic transformation of Haemophilus influenzae.
