Sequence determination and comparison of the exfoliative toxin A and toxin B genes from Staphylococcus aureus.
AUTOR(ES)
Lee, C Y
RESUMO
The DNA encoding the exfoliative toxin A gene (eta) of Staphylococcus aureus was cloned into bacteriophage lambda gt11 and subsequently into plasmid pLI50 on a 1,391-base-pair DNA fragment of the chromosome. Exfoliative toxin A is expressed in the Escherichia coli genetic background, is similar in length to the toxin purified from culture medium, and is biologically active in an animal assay. The nucleotide sequence of the DNA fragment containing the gene was determined. The protein deduced from the nucleotide sequence is a polypeptide of 280 amino acids. The mature protein is 242 amino acids. The DNA sequence of the exfoliative toxin B gene was also determined. Corrections indicate that the amino acid sequence of exfoliative toxin B is in accord with chemical sequence data.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213685Documentos Relacionados
- Sequence of the exfoliative toxin B gene of Staphylococcus aureus.
- Cloning and expression of the exfoliative toxin B gene from Staphylococcus aureus.
- Purification and characterization of different types of exfoliative toxin from Staphylococcus aureus.
- Isolation of extrachromosomal deoxyribonucleic acid for exfoliative toxin production from phage group II Staphylococcus aureus.
- Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus.