Sequence-specific targeting of MSL complex regulates transcription of the roX RNA genes
AUTOR(ES)
Bai, Xiaoying
RESUMO
In Drosophila, dosage compensation is controlled by the male-specific lethal (MSL) complex consisting of at least five proteins and two noncoding RNAs, roX1 and roX2. The roX RNAs function in targeting MSL complex to the X chromosome, and roX transgenes can nucleate spreading of the MSL complex into flanking chromatin when inserted on an autosome. An MSL-binding site (DHS, DNaseI hypersensitive site) has been identified in each roX gene. Here, we investigate the functions of the DHS using transgenic deletion analyses and reporter assays. We find that MSL interaction with the DHS counteracts constitutive repression at roX1, resulting in male-specific expression of roX1 RNA. Surprisingly, the DHS is not required for initiation of cis spreading of MSL complex, instead local transcription of roX RNAs correlates with extensive spreading.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=514957Documentos Relacionados
- The roX genes encode redundant male-specific lethal transcripts required for targeting of the MSL complex
- Local spreading of MSL complexes from roX genes on the Drosophila X chromosome
- Sequence-specific Rho–RNA interactions in transcription termination
- Sequence-specific interaction of U1 snRNA with the SMN complex
- Hedgehog signaling regulates transcription through cubitus interruptus, a sequence-specific DNA binding protein