Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus.

AUTOR(ES)

Zweig, M

RESUMO

Guinea pig antisera and mouse monoclonal antibodies against a 40,000-molecular-weight nucleocapsid protein (p40) of herpes simplex virus types 1 and 2 immunoprecipitated 40,000- and 80,000-molecular-weight classes of soluble proteins from infected cell extracts. The soluble 40,000-molecular-weight protein class (intracellular p40) appeared as a cluster of three to four closely spaced bands of proteins having molecular weights ranging between 39,000 and 45,000, whereas the soluble 80,000-molecular-weight protein class (intracellular p80) appeared as a doublet of bands. The peptide map of intracellular p40 closely resembled the maps of the p40 and p45 proteins of nucleocapsids, but it showed both differences and similarities when compared with the peptide map of intracellular p80. Pulse-chase experiments suggested that intracellular p80 was not a precursor of intracellular p40. We conclude that the intracellular p40 and p80 protein classes share common antigenic determinants, presumably reflecting similar amino acid sequences, although they have distinct differences in protein structure.

Documentos Relacionados

• Phosphorylation of ribosomal proteins in hamster fibroblasts infected with pseudorabies virus or herpes simplex virus.
• Polysome-associated proteins in herpes simplex virus-infected cells.
• Use of synthetic peptides to map the antigenic determinants of glycoprotein D of herpes simplex virus.
• Cooperative effects between two acyclovir resistance loci in herpes simplex virus.
• Effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus.