Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus.
AUTOR(ES)
Datta, A R
RESUMO
Two cosmid cloning vectors containing lambda cos sequences and a 42-base-pair multipurpose cloning sequence were constructed. pAD22 also contains a 1.4-kilobase TRP-ARS fragment from Saccharomyces cerevisiae. These cosmids transformed Escherichia coli and S. cerevisiae cells and could be mobilized into Vibrio parahaemolyticus strains with a conjugative plasmid, pRK2013. The cosmid pAD22 was genetically and structurally stable during passage through V. parahaemolyticus and E. coli strains.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214813Documentos Relacionados
- Genetic and physical characterization of proBA genes of the marine bacterium Vibrio parahaemolyticus.
- Panophthalmitis caused by Vibrio parahaemolyticus.
- Cloning of the thermostable direct or Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus.
- Membrane filter procedure for enumeration of Vibrio parahaemolyticus.
- Evaluation of four methods for enumeration of Vibrio parahaemolyticus.
