Sialylation of terminal saccharides of glycoconjugates expressed by murine molar tooth germs developing in vitro and in vivo.

AUTOR(ES)

Jowett, A K

RESUMO

During development of the mammalian tooth germ the pattern of terminal saccharides of glycoconjugates changes, with many structures losing lectin reactivity in a consistent pattern. This study investigated whether the epitopes are lost or become masked by terminal sialylation, using a combination of neuraminidase treatment of sections and sialic acid-reactive lectins. The results suggested that most of the terminal galactosamine and fucose sites in the epithelial enamel organ were removed during morphogenesis. Conversely, during condensation of the dental mesenchyme, masked peanut agglutinin (PNA)-reactive galactose epitopes appeared. During differentiation and organisation of the mesenchyme into odontoblasts and a subodontoblastic layer the PNA-reactive sites became masked again. These regions also specifically expressed sialylated glucosamine. However, at the proliferating epithelial cervical loop galactose sites appeared to be masked. This was more pronounced during in vitro development when abnormal expression of PNA-reactive sites was found at the cervical loop. Additionally, fucosylated sites persisted in the enamel organ, further indicating that the expression of terminal saccharides was disrupted during development in the organ culture system. These data suggest that loss of terminal galactose and galactosamine is related to differentiation of the cells. However, whether this loss occurs by removal or sialic acid masking is not dependent either on the origin of the cells or the epitope being lost.

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