Significance of Host Cell Kinesin in the Development of Chlamydia psittaci
AUTOR(ES)
Escalante-Ochoa, Cristina
FONTE
American Society for Microbiology
RESUMO
The influence of the microtubule-associated motor protein kinesin on Chlamydia psittaci inclusion development in epithelial and fibroblast cell lines was addressed. Kinesin was blocked early after chlamydial internalization (4 h postinfection [p.i.]) and before the initiation of active chlamydial multiplication (8 h p.i.). Chlamydia development was monitored by fluorescence and transmission electron microscopy at different times during the cycle. In both host cell lines, kinesin blockage restricted mitochondria from the chlamydial vacuole. The effects of kinesin blockage on the C. psittaci replication cycle included the presence of multiple inclusions up to late in the cycle, the presence of enlarged pleomorphic reticulate bodies, and a delayed reappearance of elementary bodies. The last effect seems to be greater when kinesin is blocked early after infection. Our results show that kinesin activity is required for optimal development of these microorganisms, most probably acting through the apposition of mitochondria to the C. psittaci inclusions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=96902Documentos Relacionados
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