Simian virus 40 functions required for the establishment and maintenance of malignant transformation.
AUTOR(ES)
Martin, R G
RESUMO
Members of the five classes of temperature-sensitive simian virus 40 mutants were tested for their ability to transform Chinese hamster lung cells. Two criteria for transformation were used: the ability to form clones in medium with low serum concentrations and the ability to overgrow a monolayer. Only A mutants failed to transform at the restrictive temperature when subconfluent Chinese hamster lung monolayers were used. However, both A and D mutants failed to transform at the restrictive temperature when confluent monolayers and depleted medium were used. When transformed clones were selected, purified by recloning, and examined at both temperatures, only cell lines induced by A mutants lost the transformed phenotype at the higher temperature. Thus, A function is required for maintenance of the transformed phenotype in Chinese hamster lung cells. A function is known to be required for the initiation of viral DNA synthesis in permissive cells. Therefore, transformation may be a consequence of the introduction into a cell of the capacity for aberrant initiation of DNA replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354496Documentos Relacionados
- Simian virus 40 gene A function and maintenance of transformation.
- New region of the simian virus 40 genome required for efficient viral transformation.
- Structural prerequisites of simian virus 40 large T antigen for the maintenance of cell transformation.
- Role of simian virus 40 gene A function in maintenance of transformation.
- Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.