Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.
AUTOR(ES)
Cabib, E
RESUMO
After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=236077Documentos Relacionados
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