Simple phagemid-based system for generating allele replacements in Escherichia coli.
AUTOR(ES)
Slater, S
RESUMO
We describe a phagemid-based system for rapidly generating chromosomal allele replacements in two steps. The system utilizes simple vector-determined selection procedures for each step and requires only that the target strain be male and sup+ (nonsuppressing for amber mutations).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204859Documentos Relacionados
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