Simplified Assay for Concanavalin A-Dependent Bacterial Agglutination by Using Cell Surface Mutants

AUTOR(ES)

Maruyama, H. B.

RESUMO

Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A.

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