Simplified method for typing herpes simplex virus by restriction endonuclease analysis.
AUTOR(ES)
Arens, M Q
RESUMO
We have developed a simplified method for unambiguously typing herpes simplex virus. The method depends on the production of cell-associated virus at 34 degrees C and subsequently, on the separation of cellular DNA and viral DNA by Dounce homogenization and the removal of nuclei by centrifugation. Viral nucleic acid was prepared from the cytoplasmic fraction and analyzed after restriction endonuclease cleavage. The method obviates the use of radioactive isotopes, and the viral DNA is effectively free of interfering cellular DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272683Documentos Relacionados
- Simplified restriction endonuclease method for typing and subtyping adenoviruses.
- Comparison of a commercial ELISA system with restriction endonuclease analysis for typing herpes simplex virus.
- Typing of herpes simplex virus by capture biotin-streptavidin enzyme-linked immunosorbent assay and comparison with restriction endonuclease analysis and immunofluorescence method using monoclonal antibodies.
- Unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis.
- A miniaturised and simplified technique for typing and subtyping herpes simplex virus.