Single nisoldipine-sensitive calcium channels in smooth muscle cells isolated from rabbit mesenteric artery.
AUTOR(ES)
Worley, J F
RESUMO
Single smooth muscle cells were enzymatically isolated from the rabbit mesenteric artery. At physiological levels of external Ca, these cells were relaxed and contracted on exposure to norepinephrine, caffeine, or high levels of potassium. The patch-clamp technique was used to measure unitary currents through single channels in the isolated cells. Single channels were selective for divalent cations and exhibited two conductance levels, 8 pS and 15 pS. Both types of channels were voltage-dependent, and channel activity occurred at potentials positive to -40 mV. The activity of both channel types was almost completely inhibited by 50 nM nisoldipine. These channels appear to be the pathways for voltage-dependent Ca influx in vascular smooth muscle and may be the targets of the clinically used dihydropyridines.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=386366Documentos Relacionados
- Calcium-activated potassium channels in single smooth muscle cells of rabbit jejunum and guinea-pig mesenteric artery.
- Noradrenaline modulation of calcium channels in single smooth muscle cells from rabbit ear artery.
- ATP-activated channels gate calcium entry in single smooth muscle cells dissociated from rabbit ear artery.
- Exchange characteristics of the noradrenaline-sensitive calcium store in vascular smooth muscle cells or rabbit ear artery.
- Action of histamine on single smooth muscle cells dispersed from the rabbit pulmonary artery.
