Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)

AUTOR(ES)

Kumar, Dinesh, Gupta, Neelam, Ahlawat, S.P.S., Satyanarayana, R., Sunder, Shyam, Gupta, S.C.

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

2006

RESUMO

The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect a-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.

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