Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
AUTOR(ES)
Winans, S C
RESUMO
A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215030Documentos Relacionados
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