Site-specific DNA binding of nuclear factor I: effect of the spacer region.
AUTOR(ES)
Gronostajski, R M
RESUMO
Nuclear factor I (NFI) is a site-specific DNA binding protein required for the replication of adenovirus type 2 DNA in vitro and in vivo. To study sequence requirements for the interaction of NFI with DNA, we have measured the binding of the protein to a variety of synthetic sites. Binding sites for NFI (FIB sites) were previously shown to contain a consensus sequence composed of 2 motifs, TGG (Motif 1), and GCCAA (Motif 2), separated by a 6 or 7bp spacer region. To assess conserved sequences in the spacer region and flanking sequences which affect NFI binding, we have isolated clones from oligonucleotide libraries that contain the two motifs flanked by 3 degenerate nucleotides and separated by degenerate spacer regions of 6 or 7 nucleotides. With a 6bp spacer region, a strong bias exists for a C or A residue in the first position of the spacer. Sites with a 7bp spacer region contain a G and C or A residue at the first and second positions, respectively, of the spacer, but also possess conserved residues at other positions of the site.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=306006Documentos Relacionados
- Site-specific DNA binding of nuclear factor I: analyses of cellular binding sites.
- Site-specific carcinogen binding to DNA.
- The role of the loxP spacer region in P1 site-specific recombination.
- Site-specific antibodies to human erythropoietin directed toward the NH2-terminal region.
- Site-specific initiation of DNA replication within the non-transcribed spacer of Physarum rDNA.