Specialized Transducing Phages Derived from Phage P22 That Carry the proAB Region of the Host, SALMONELLA TYPHIMURIUM: Genetic Evidence for Their Structure and Mode of Transduction

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RESUMO

Two independently isolated specialized transducing phages, P22 pro-1 and P22pro-3, have been studied. Lysates of P22pro-1 contain a majority of transducing phages which can go through the lytic cycle only in mixed infection; these defective phages transduce by lysogenization in mixed infection and by substitution in single infection. A few of the transducing phages in P22pro-1 lysates appear to be non-defective, being able to form plaques and to transduce by lysogenization in single infection. Transduction by P22pro-3 lysates is effected by non-defective transducing phages, which transduce by lysogenization; these lysates also contain a majority of defective phages which do not co-operate in mixed infection.

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