Specific deletion of DNA sequences between preselected bases.
AUTOR(ES)
Panayotatos, N
RESUMO
Blunt-end ligation of a "filled-in" HindIII, Sal I, Ava I or Bcl I restriction site with a DNA fragment having A, G, C, or T as the terminal 3' nucleotide regenerates the corresponding restriction site. A combination of this property with the action of BAL 31 nuclease which progressively removes base-pairs from the ends of linear DNA, can generate deletions extending to desired pre-selected nucleotides, and introduces unique restriction sites at those positions. Similarly other restriction sites can be used to select for the deletion of sequences between specific di-, tri-, tetra- and penta-nucleotides. Using this method, 10 base pairs were deleted from the end of a restriction fragment carrying the late promoter for bacteriophage T7 gene 1.1, to create a molecule with a unique restriction site at the initiation codon for translation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327552Documentos Relacionados
- Tetraplex folding of telomere sequences and the inclusion of adenine bases.
- Rescue of abasic hammerhead ribozymes by exogenous addition of specific bases.
- Modification of interactions between neutrophils and staphylococci by lysosomotropic weak bases.
- Conformational properties of Z-forming DNA oligomers bearing terminal unpaired bases.
- Specific tandem GG to TT base substitutions induced by acetaldehyde are due to intra-strand crosslinks between adjacent guanine bases.
