Specific transcription of cloned Methanobacterium thermoautotrophicum transcription units by homologous RNA polymerase in vitro.
AUTOR(ES)
Knaub, S
RESUMO
Specific in vitro transcription by partially purified RNA polymerase from Methanobacterium thermoautotrophicum of DNA sequences cloned in between the promoter and terminator regions of the methyl CoM reductase transcription unit of the same organism is described. The 5'-terminus of the product has been mapped. Deletion analyses of the promoter region show the limits of the sequences essential for the promoter function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=330509Documentos Relacionados
- Methanobacterium thermoautotrophicum RNA Polymerase and Transcription In Vitro
- Accurate transcription of cloned Neurospora RNA polymerase II-dependent genes in vitro by homologous soluble extracts.
- Accurate transcription of cloned Neurospora RNA polymerase II-dependent genes in vitro by homologous soluble extracts
- In vitro transcription by purified yeast RNA polymerase II. Coarse promoter mapping on homologous cloned genes.
- RNA polymerase II ternary transcription complexes generated in vitro.
