Specificity of Hpa II and Hae III DNA methylases

AUTOR(ES)

Mann, Michael B.

RESUMO

The methylases M·HaeIII and M·HpaII recognize the tetranucleotide sequences [Formula: see text] and [Formula: see text] respectively, in DNA, and transfer a methyl group from S-adenosylmethionine to the 5-position of cytosine on each strand as indicated by the asterisks. Restriction endonuclease R·HaeIII does not cleave the methylated sequence [Formula: see text] but can cleave [Formula: see text] in which methylation is introduced on the unnatural external cytosine positions. Similarly, R·HpaII does not cleave [Formula: see text] but can cleave [Formula: see text].

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